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OriginLab corp
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Image Search Results
Journal: Rsc Applied Polymers
Article Title: Matrix metalloproteinase responsive hydrogel microplates for programmed killing of invasive tumour cells
doi: 10.1039/d3lp00057e
Figure Lengend Snippet: Cytotoxicity on cancer cells. (a) FRET probe based collagenase IV (MMP-2/9) enzymatic activity assay, conducted with culture media after 24 h culture with cells, compared to 10 nM collagenase IV control solution (inhibitor from supplied kit, Merck InnoZyme), (b) MTT assays of various microplate treatment options (U87-MG cells) at 72 h (non-loaded microplates at the same particle concentration as used in the DTXL containing samples), (c) MTT assays of various microplate treatment options (U87-MG cells) at 72 h, (d) fitted dose response curve EC 50 values from various treatment groups, (e) brightfield microscopy images of U87-MG (7500 cells per well), after 48 h of exposure to MMP degradable (top) or non-degradable (bottom) μPL containing DTXL SPNs, EMEM medium, 100 nM total DTXL concentration. Scale bars: 100 μm.
Article Snippet: EC 50 values were calculated by
Techniques: Enzyme Activity Assay, Control, Concentration Assay, Microscopy